indirectelisaprotocol

由 T Waritani 著作 · 2017 · 被引用 119 次 — To assay serum antibodies by indirect ELISA, it is critical to eliminate a variety of false positive and negative reactions attributed to ...

Dilute purified antigens to a final concentration of 3.0 μg/ml in antigen-coating buffer and add 50 μl of diluted antigen to each well of a 96-well ELISA plate.

Incubate with Primary Antibody · Add 100 uL of the appropriately diluted primary antibody to each well. · Seal the plate and incubate for 1 hour at 37°C or 2 ...

In an indirect ELISA, antigen is immobilized, then a specific antibody detects it; a secondary, enzyme conjugated, antibody together with a substrate ...

由 TO Kohl 著作 · 2017 · 被引用 46 次 — 1. Dilute the antigen to a final concentration of 1 µg/mL for protein or 0.1 µg/mL for peptides in PBS, carbonate buffer, or TBS. Coat the wells of a microtiter ...